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Cary 3500 Multicell UV-Vis Spectrophotometer

Cary 3500 Multicell UV-Vis Spectrophotometer

The Cary 3500 Multicell UV-Vis is a double beam spectrophotometer offering new, unique measurement capabilities that can be deployed in regulated environments. The system simultaneously measures up to four independent temperature experiments across eight cuvette positions, with air-cooled Peltier temperature control. A xenon flash lamp—complete with 10-year replacement warranty (for Cary 3500 instruments purchased from Agilent or participating partners)—eliminates the daily warm-up time and drastically reduces the frequency and cost of lamp replacement. You can also accurately monitor kinetic experiments with a data collection rate up to 250 points per second.

The Cary 3500 comes complete with Cary UV Workstation software—a next-generation analytical platform with streamlined methods for wavelength reads, scanning, concentration, kinetics, and temperature measurements. When configured with Agilent OpenLab data management software, Cary UV Workstation data is managed, protected, and stored in a local or central secure database.

Cary 3500 Multicell UV-Vis Spectrophotometer

Cary 3500 Multicell UV-Vis Spectrophotometer

Product Details

Features
  • Measure samples at four temperatures across eight cuvette positions in a single experiment and collect data from each cuvette simultaneously.
  • Control the temperature of your samples accurately and quickly from 0 to 110 °C without water, noise, or messy cables using a unique and fully integrated air-cooled Peltier system.
  • Control UV-Vis thermal ramp measurements at up to 40 °C/min using highly accurate, solid-state digital Cary temperature probes that control experimental temperature from inside the cuvette.
  • Ensure analytical accuracy for small-volume measurements with a highly-collimated and uniform beam of less than 1.5 mm width, coupled with permanent optical alignment of the stationary cell holders.
  • Reduce data processing time and calculation errors with more than 50 built-in and customized calculations that can be saved and applied automatically to any method used routinely.
  • Achieve research grade photometric performance, stability, and range with a double beam spectrophotometer featuring leading optical technologies.
  • Be up and running in minutes by following the intuitive video tutorials in Cary UV Workstation’s Help and Learning Center.
  • Meet data integrity standards—provides the technical controls to securely acquire, process, report and store data in laboratories that must follow the compliance guidelines of FDA 21 CFR Part 11, EU Annex 11, GAMP5, as well as ISO/IEC 17025 and EPA’s 40 CFR Part 160.
  • Store and manage your Cary 3500 Multicell UV-Vis spectrophotometer data in a local secured storage on the workstation PC, or in a central storage location.
  • Deploy the OpenLab software suite and get optional secure database storage for electronic records, complete administration for users and access privileges, software licensing, and security settings—e-signature workflow and enhanced audit trail review capabilities.
  • Choose sustainability: The Cary 3500 Multicell UV-Vis received My Green Lab’s ACT (Accountability, Consistency, Transparency) label after independent audit for its environmental impact throughout the product lifecycle.
Specifications
Compliance Software
  • OpenLab Software Suite
Control Software
  • Cary UV Workstation
  • Cary 3500 Multizone Add-on
Depth
  • 700 mm
Height
  • 280 mm
Light Source
  • Xenon flash lamp (250 Hz) with 10-year warranty on new instruments
Maximum Scanning Speed
  • UV-Vis 150,000 nm/min
Photometric Range (Abs)
  • 4 Abs
Photometric System
  • Double beam
Power Requirements
  • 100-240 VAC, 50/60 Hz
Spectral Bandwidth
  • UV-Vis 0.1 – 5.0 nm
Wavelength
  • 190 – 1100 nm
Weight
  • 34 kg
Width
  • 445 mm
Z-height
  • 15 mm
How It Works

Streamline your experimental design and amplify confidence in your results

The Cary 3500 Multicell UV-Vis spectrophotometer scans the full wavelength range in less than a second, on all eight channels—simultaneously. The system is available in two configurations, ambient or temperature-controlled, tuned to your application needs. The temperature-controlled configuration comes with an air-cooled Peltier system. With the multizone software add-on, each pair of cuvettes can be held at a different temperature—so you can do four experiments at once.

Streamline your UV-Vis experiments

The Cary 3500 UV-Vis is powered by a xenon flash lamp requiring no warmup, with full spectrum scans collected in less than a second. With no moving parts, your results are accurate and reproducible—every time—even when working with very low sample volumes.

A built-in Help and Learning Center reduces training time, with video guidance for new or infrequent users. Plus, with its small footprint and robust design, the Cary 3500 requires minimal maintenance.

Cary UV Workstation—intuitive and secure UV-Vis software

The Cary UV Workstation software comes with built-in applications such as kinetics, concentration, wavelength scanning, and temperature-based measurements. It is compatible with the Agilent OpenLab software suite of products to help you meet global regulatory requirements.

Learn more about Cary 3500 and Data Integrity Options for GMP Facilities.

Environmental impact

The Cary 3500 Multicell UV-Vis spectrophotometer has been independently audited for its environmental impact and has received the ACT (Accountability, Consistency, Transparency) label, published by My Green Lab.  See how you can reduce your lab’s environmental impact with sustainability-driven innovation.

Applications

Quantifying a molecule—concentration measurements

QC processes in the pharmaceutical industry often use this reliable, well-established approach, and data integrity is crucial. The Agilent Cary 3500 Multicell UV-Vis spectrophotometer measures samples, standards, and controls simultaneously, eliminating most analytical variables.​

Conformational studies of proteins and DNA/RNA

Structural or conformational transitions are often associated with changes in UV-Vis absorbance. UV-Vis spectroscopy can therefore be used to study conformational changes in molecules such as proteins and DNA/RNA.​ UV-Vis is often used in protein and nucleic acid thermal melt analyses, and sample temperature control is key in ID testing. Read the application note and case study to see how the Cary 3500 UV-Vis allows accurate, reproducible ramping up to 40 °C/min. Or browse our comprehensive guide to thermal melt analysis by UV-Vis.​

Monitoring reaction kinetics with UV-Vis

UV-Vis is ideal for analyzing reaction kinetics when the change in reactant or products produces a change in absorbance at a specific wavelength over time. Single point kinetics monitors a single wavelength, scanning kinetics monitors a wavelength range. The Cary 3500 Multicell UV-Vis collects data quickly (up to 250 data point per second) —perfect for scanning kinetics measurements. Watch the video to see how the Cary 3500 measures multiple samples at different temperatures, simultaneously.​

Technology

Xenon Flash Lamps for UV-Vis & Fluorescence Spectrophotometers

For more than 35 years, Agilent has been the pioneer in xenon flash lamp technology for UV-Vis and fluorescence instrumentation. Agilent provides innovative workflow solutions using xenon lamps, including our 10-year lamp warranty, on Cary 60 UV-Vis, Cary 3500 UV-Vis, and Cary Eclipse Fluorescence spectrophotometers.

UV-Vis Analysis of Biomolecules

Analyzing proteins, oligos, monoclonal antibodies, and other biomolecules? UV-Vis spectroscopy is a key part of any bioanalytical toolkit, with many features and benefits that accelerate workflows involving biomolecules. Read our guide and customer case studies to learn more.

Double Beam Spectrophotometers

Double beam spectrophotometers are fast and accurate, using two beams of light—a sample beam and a reference beam—to correct instrument fluctuations in real-time.

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